IJPPP Copyright © 2009-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Physiol Pathophysiol Pharmacol 2010;2(1):64-68.

Brief Communication
Real-world exposure of airborne particulate matter triggers oxidative stress in an
animal model

Guohui Wang, Sanjay Rajagopalan, Qinghua Sun, Kezhong Zhang

Center for Molecular Medicine & Genetics, Department of Immunology and Microbiology, The Wayne State University School of
Medicine, Detroit, MI 48201, USA; Division of Cardiovascular Medicine, Davis Heart & Lung Research Institute, College of Medicine,
Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH 43210, USA

Received February 21, 2010, accepted March 5, 2010, available online March 15, 2010

Abstract: Epidemiological studies have shown strong link between air pollution and the increase of cardio-pulmonary mortality and
morbidity. In particular, inhaled airborne particulate matter (PM) exposure is closely associated with the pathogenesis of air pollution-
induced systemic diseases. In this study, we exposed C57BL/6 mice to environmentally relevant PM in fine and ultra fine ranges
(diameter < 2.5 µm, PM2.5) using a "real-world" airborne PM exposure system.  We investigated the pathophysiologic impact of PM2.5
exposure in the animal model and in cultured primary pulmonary macrophages. We demonstrated that PM2.5 exposure increased the
production of reactive oxygen species (ROS) in blood vessels in vivo.  Furthermore, in vitro PM2.5 exposure experiment suggested that
PM2.5 could trigger oxidative stress response, reflected by increased expression of the anti-oxidative stress enzymes superoxide
dismutase-1 (SOD-1) and heme oxygenase-1(HO-1), in mouse primary macrophages. Together, the results obtained through our “real-
world” PM exposure approach demonstrated  the pathophysiologic effect of ambient PM2.5 exposure on triggering oxidative stress in
the specialized organ and cell type of an animal model. Our results and approach will be informative for the research in air pollution-
associated physiology and pathology. (IJPPP1002002).

Key words: Air pollution; airborne particulate matter; oxidative stress; reactive oxygen species; animal model

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Address all correspondence to:
Kezhong Zhang, PhD
540 E. Canfield Avenue
Detroit, MI 48201
Tel: 313-577-2669; FAX: 313-577-5218
Email:
kzhang@med.wayne.edu

Qinghua Sun, MD, PhD
396 Biomedical Research Tower
Columbus, OH 43210
Tel: 614-247-1560; Fax: 614-688-4233
E-mail:
sun.224@osu.edu