IJPPP Copyright © 2009-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Physiol Pathophysiol Pharmacol 2011;3(1):38-47.

Original Article
Expression of neurogenesis genes in human temporal lobe epilepsy with
hippocampal sclerosis

Tobias Engel, Clara K. Schindler, Amaya Sanz-Rodriguez, Robert Meller, Roger P. Simon, David C. Henshall

Department of Physiology & Medical Physics, Royal College of Surgeons in Ireland, Dublin 2, Ireland. Robert S. Dow Neurobiology
laboratories, Legacy Research, Portland, Oregon, USA. Neuroscience Institute, Morehouse School of Medicine, Atlanta, GA, USA.

Received November 6, 2010; accepted November, 2009; Epub November , 2010; Published January 1, 2011

Abstract: Both evoked and spontaneous seizures have been reported to increase neurogenesis in animal models. Less is known
about whether neurogenesis and markers thereof are aberrantly expressed in human temporal lobe epilepsy (TLE) with hippocampal
sclerosis. In the present study we measured protein levels of multiple neurogenesis marker genes in tissue homogenates of sclerotic
hippocampus surgically resected from patients with pharmacoresistant TLE (n = 7). Results were compared to a group of age- and
gender-matched autopsy control hippocampi (n = 6). Expression of the mature neuron marker NeuN was significantly lower in TLE
samples compared to controls. In contrast, levels of neurogenesis-associated genes including TUC-4, doublecortin, NeuroD and
Numb, were all similarly expressed in TLE and control hippocampus samples. The present study suggests hippocampal expression
levels of proteins associated with neurogenesis are not notably different in human TLE, implying the sclerotic hippocampus may retain
neurogenic potential. (IJPPP1011001).

Key words: Bromodeoxyuridine, epileptogenesis, neurogenesis, mesial temporal sclerosis, seizure

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Address all correspondence to:
David C. Henshall, PhD
Department of Physiology & Medical Physics
Royal College of Surgeons in Ireland
123 St. Stephen’s Green
Dublin 2, Ireland.
Tel: +35314028629,  Fax: +35314022447
E-mail: dhenshall@rcsi.ie