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Int J Physiol Pathophysiol Pharmacol 2011;3(2):71-87.

Original Article
Localization of gentamicin uptake in the acutely isolated inner ear of the rat

Katharina Schmid, Jürgen Strutz, Otto Gleich, Pingling Kwok

Department of Otolaryngology - Head and Neck Surgery, University of Regensburg, Franz-Josef-Strauß-Allee 11, D-93042 Regensburg,
Germany

Received February 25, 2011; accepted March 23, 2011; Epub March 28, 2010; Published June 30, 2011

Abstract: The quantitative analysis of fluorescence in frozen sections of rat inner ears exposed to Texas Red conjugated gentamicin
revealed distinct gradients of gentamicin fluorescence. At 500 µg/ml gentamicin fluorescence  occurred in inner and outer hair cells, the
interdental cell region, the spiral limbus below the interdental cells, the nerve fiber bundle in the spiral lamina, the inner sulcus cells
and the dorsal region of the spiral ligament. No gentamicin fluorescence was observed in the Hensen / Claudius cells, the ventral
region of the spiral ligament, the stria vascularis and the spiral ganglion. In the vestibule only the hair cell epithelium and the
transitional cells of the saccule showed gentamicin fluorescence  while no gentamicin fluorescencewas found in hair cell epithelia and
transitional cells of utricle and ampule, nerve fibers below hair cell epithelia of saccule, utricle and ampule and in dark cells. The
gentamicin flurescence gentamicin increased at higher concentrations. Gentamicin exposure led to more pronounced gentamicin
fluorescence in the cochlea compared to the vestibule. Based on the predominant gentamicin fluorescence in the hair cell - limbus
region of the cochlea at a low dose we propose that gentamicin may interact with the K+-flow from the inner hair cells back to the scala
media. (IJPPP1102003).

Key words: Gentamicin, Menière’s disease, cochlea, vestibule, rat

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Address all correspondence to:
Dr. Katharina Schmid
Department of Otolaryngology, Head and Neck Surgery
University of Regensburg, D-93053
Regensburg, Germany.
Tel: +49-941-944-9410, Fax: +49-941-944-9431
E-mail: k
atharina.schmid@klinik.uni-regensburg.de