IJPPP Copyright © 2009-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Physiol Pathophysiol Pharmacol 2012;4(2):108-114.

Original Article
Roles of oxidative stress in synchrotron radiation X-ray-induced testicular damage
of rodents

Yingxin Ma*, Hui Nie*, Caibin Sheng, Heyu Chen, Ban Wang, Tengyuan Liu, Jiaxiang Shao, Xin He, Tingting Zhang, Chaobo Zheng,
Weiliang Xia, Weihai Ying

School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, P.R. China.
*Contributed equally.

Received July 31, 2011; accepted May 17, 2012; Epub June 20, 2012; Published June 30, 2012

Abstract: Synchrotron radiation (SR) X-ray has characteristic properties such as coherence and high photon flux, which has excellent
potential for its applications in medical imaging and cancer treatment. However, there is little information regarding the mechanisms
underlying the damaging effects of SR X-ray on biological tissues. Oxidative stress plays an important role in the tissue damage
induced by conventional X-ray, while the role of oxidative stress in the tissue injury induced by SR X-ray remains unknown. In this study
we used the male gonads of rats as a model to study the roles of oxidative stress in SR X-ray-induced tissue damage. Exposures of
the testes to SR X-ray at various radiation doses did not significantly increase the lipid peroxidation of the tissues, assessed at one day
after the irradiation. No significant decreases in the levels of GSH or total antioxidation capacity were found in the SR X-ray-irradiated
testes. However, the SR X-ray at 40 Gy induced a marked increase in phosphorylated H2AX – a marker of double-strand DNA damage,
which was significantly decreased by the antioxidant N-acetyl cysteine (NAC). NAC also attenuated the SR X-ray-induced decreases in
the cell layer number of seminiferous tubules. Collectively, our observations have provided the first characterization of SR X-ray-induced
oxidative damage of biological tissues: SR X-ray at high doses can induce DNA damage and certain tissue damage during the acute
phase of the irradiation, at least partially by generating oxidative stress. However, SR X-ray of various radiation doses did not increase
lipid peroxidation.

Keywords: Synchrotron radiation, X-ray, oxidative stress, testes, DNA damage, lipid peroxidation


Address all correspondence to:
Dr. Weihai Ying, School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road,
Shanghai, 200030, P.R. China Tel: 011-86-21-6293-3075; Fax: 011-86-21-6293-2302; E-mail: weihaiy@sjtu.edu.cn. Dr. Weiliang Xia,
School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai,
200030, P.R. China Tel: 011-86-21-6293-3291; Fax: 011-86-21-6293-2302; E-mail: wlxia@sjtu.edu.cn