IJPPP Copyright © 2009-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Physiol Pathophysiol Pharmacol 2012;4(1):51-58.

Original Article
Rat model of food-induced non-obese-type 2 diabetes mellitus: comparative
pathophysiology and histopathology

Akindele O Adeyi, Babatunde A Idowu, Chiedu F Mafiana, Samuel A Oluwalana, Oluwasola L Ajayi, Oluseyi A Akinloye

Department of Zoology, University of Ibadan, Ibadan, Nigeria; Department of Biological Sciences, University of Agriculture, Abeokuta,
Nigeria; Office of the Executive Secretary, National Universities Commission, Abuja, Nigeria; Department of Forestry and Wildlife
Management, University of Agriculture, Abeokuta; Department of Veterinary Pathology, University of Agriculture, Abeokuta, Nigeria;
Department of Biochemistry, University of Agriculture, Abeokuta, Nigeria

Received January 26, 2012; accepted February 23, 2012; Epub February 28, 2012; Published March 15, 2012

Abstract: Based on the hypothesis that consistent hyperglycemia can result in insulin resistance, we explored the induction of NIDDM
using diet of high glycemic/low fat index and compared the effects on the physiology and histology of the rats. The rats were in 3 groups
of 20 rats each. DM was induced in the first group by single intraperitoneal injection of 150mg/kg alloxan monohydrate and in the
second group by feeding the rats with diet of high glycemic index/low fat for 8 weeks. The pathophysiology and histopathology of DM
were studied. Hyperglycemia was recorded in the alloxan and food-induced groups respectively. Both groups were also positive for
glycosuria, which confirmed the induction of DM. Concentrations of plasma potassium, calcium, protein and urea were higher (p<0.05)
in the alloxan-induced than the food-induced rats, whereas food-induced rats recorded higher hematological indices than the
alloxan-induced group. Coronary risk indices were higher in food-induced rats than the alloxan-induced, while activities of antioxidant
enzymes were significantly higher (p<0.05) in alloxan-induced diabetic rats than the food-induced rats. Marked degenerations of the
Islets of Langerhans was observed in pancreas of alloxan-induced diabetic rats, whereas, histological examination of the pancreas of
food-induced and control rats revealed no visible lesion. Liver and kidney of all food and alloxan-induced diabetic rats showed marked
degeneration of the hepatocytes and the glomeruli respectively. This study presents a rat model of type II diabetes mellitus using food
of high glycemic/low fat index with its consequent ionoregulatory disruptions, acute anemia, hyperlipidemia, nephropathy and
hepatopathy. (IJPPP1201004).

Keywords: Diabetes mellitus, alloxan-induced, food-induced, pathophysiology, histopathology

Address all correspondence to:
Dr. Akindele O Adeyi
Department of Zoology, University of Ibadan
Ibadan, Nigeria.
E-mail: delegenius@yahoo.com