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Int J Physiol Pathophysiol Pharmacol 2012;4(3):167-173

Original Article
Effects of 5/6 nephrectomy on renal function and blood pressure in mice

Agata L Gava, Flavia PS Freitas, Camille M Balarini, Elisardo C Vasquez, Silvana S Meyrelles

Laboratory of Transgenes and Cardiovascular Control, Physiological Sciences Graduate Program, Health Sciences Center, Federal
University of Espirito Santo, Vitoria, ES, Brazil; Emescam College of Health Sciences, Vitoria, ES, Brazil; Biotechnology Graduate
Program, Health Sciences Center, Federal University of Espirito Santo, Vitoria, ES, Brazil

Received August 7, 2012; accepted September 7, 2012; Epub September 20, 2012; Published September 30, 2012

Abstract: Background/Aims: The rat has been the most used experimental animal for studies of cardiovascular and kidney diseases.
However, it is notable that there is increasing importance placed on the use of the mouse model to increase understanding of these
pathophysiologies. The aim of the present study was to induce chronic kidney disease in a mouse model and to evaluate the resulting
changes in blood pressure (BP) and in renal morphology and function. Methods: Adult male C57BL/6 mice underwent 5/6 nephrectomy
(5/6 Nx) or a sham operation (Sham). Two weeks later, conscious animals were subjected to a 24-hour urine collection and to a direct
measurement of BP. Results: Compared to Sham animals, 5/6 Nx mice showed reduced creatinine clearance (3-fold, p<0.01),
proteinuria (1.5-fold, p<0.01) and uremia (4-fold, p<0.01), as well as high blood pressure (~20%, p<0.01). 5/6 Nx animals showed
increases in the 24 h urine excretion of Na+ (2-fold, p<005), K+ (~2-fold, <0.01) and Ca2+ (~12-fold). Kidney histology of 5/6 Nx mice
also demonstrated glomerular hypertrophy (1.5-fold, p<0.05), mesangial expansion (~40%, p<0.01) and increased glomerular collagen
deposition (~30%, p<0.05). Conclusion: Induction of 5/6 nephrectomy in mice for two weeks leads to systemic arterial hypertension and
to functional and morphological damage of the remnant kidney, which are considered the main characteristics of chronic kidney
disease. (IJPPP1208002).

Keywords: Chronic kidney disease, renal function, 5/6 nephrectomy, mice

Address all correspondence to:
Dr. Elisardo C Vasquez
Lab Transgenes and Cardiovascular Control
Physiological Sciences Graduate Program
Health Sciences Center, Federal University of Espirito Santo
Av Marechal Campos 1468, 29042-755 Vitoria, ES, Brazil.
Tel: (55-27) 3335-7487; Fax (55-27) 3335-7330
E-mail: evasquez@pq.cnpq.br