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Int J Physiol Pathophysiol Pharmacol 2013;5(2):102-108

Original Article
Antioxidant activity of simvastatin prevents L-arginine-induced acute toxicity of

Ismail I Matalka, Nizar M Mhaidat, Lina A Fatlawi

Department of pathology, Jordan University of Science and Technology, Irbid 22110, Jordan; Department of Clinical Pharmacy, Jordan
University of Science and Technology, Irbid 22110, Jordan

Received January 18, 2013; Accepted April 26, 2013; Epub May 27, 2013; Published June 15, 2013

Abstract: L-arginine is a semi-essential amino acid that found naturally in food. It has been shown that administration of large doses
of L-arginine can induce acute pancreatitis. In the present study, we evaluated if simvastatin, a 3-hydroxy-methylglutaryl coenzyme A
reductase (HMG-CoA reductase) inhibitor, might prevent acute pancreatitis induced by L-arginine. Thirty male Wistar rats were
randomly allocated to five groups. Groups were: DMSO, saline, simvastatin, L-arginine, and simvastatin with L-arginine. Twenty four
hours after the last dose, rats were sacrificed and their blood was collected from heart for biochemical analysis. Pancreatic tissues
were obtained for analysis of glutathione peroxidase (GPx), glutathione s-transferase (GST), lipid peroxide levels (MDA) and histology
analysis was examined for pancreas. Results indicated that treatment with simvastatin significantly enhanced levels of GPx and GST
and decreased lipid peroxide levels induced by L-arginine compared to the vehicle. Moreover, histopathological analysis further
confirmed that administration of simvastatin relatively prevented pancreatic acinar cell damage compared to those animals received
L-arginine alone. These findings pointed out the protective role of simvastatin against acute pancreatitis induced by high doses of
L-arginine. (IJPPP1301003).

Keywords: L-arginine, acute pancreatitis, simvastatin, lipid peroxidation, antioxidant enzymes

Address correspondence to: Dr. Ismail Matalka, Department of Pathology and Laboratory Medicine, School of Medicine, Jordan
University of Science and Technology, Irbid 22110, Jordan. Tel/Fax: +962-2-7200626; E-mail: imatalka@just.edu.jo;