IJPPP Copyright © 2009-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Physiol Pathophysiol Pharmacol 2013;5(4):239-247

Original Article
On the effect of the injection of potassium phosphate in vivo inducing the
precipitation of serum calcium with inorganic phosphate

Alcimar B Soares, José G Ticianeli, Letícia B M Soares, George Amaro

Laboratory of Biomedical Engineering, Federal University of Uberlândia, Uberlândia, MG, Brazil; Nephrology Service, General Hospital,
Federal University of Uberlândia, Uberlândia, MG, Brazil; Brazilian Agricultural Research Company, Boa Vista, RR, Brazil

Received October 25, 2013; Accepted November 30, 2013; Epub December 15, 2013; Published December 30, 2013

Abstract: High concentrations of inorganic phosphate (Pi) resulted from the hydrolysis of ATP is strongly associated to the weakness
of the contractile mechanism of muscles due to its attractiveness to calcium. The majority of the experiments to study such effect are
conducted in vitro. This work investigates the effects of different concentrations of Pi, induced by the injection of potassium phosphate
in live animals, in the precipitation with serum calcium and the generation of calcium phosphate composites. The experiments were
also designed to find out the ideal amount of potassium phosphate to induce an effective reaction. Potassium phosphate was injected
in Wistar rats, randomly separated and distributed into seven groups. Group I was injected with 0.5 ml of saline solution (control) and
groups II through VII were injected with 0.5, 1.5, 2.5, 5.0, 7.5 and 10.0 mg/kg of potassium phosphate, respectively. Blood collected from
the inferior vena cava was submitted to biochemical analyses to measure the concentrations of calcium, Pi, urea and creatinine. The
results showed that Pi, induced by the injection of potassium phosphate in live animals, causes precipitation with serum calcium, with
statistically significant differences between the control and the treatment groups for doses up to 5.0 mg/kg. No statistically significant
differences were found between the different doses and the concentration of urea and creatinine in the plasma. We conclude that
potassium phosphate can be used to induce serum calcium precipitation in-vivo, with minor effects on other physiological variables,
and the ideal dose to do so is 5.0 mg/kg. (IJPPP1310003).

Keywords: In vivo, potassium phosphate, inorganic phosphate, serum calcium, muscle fatigue

Address correspondence to: Dr. Alcimar B Soares, Laboratory of Biomedical Engineering, Federal University of Uberlândia,
Uberlândia, MG, Brazil. E-mail: alcimar.soares@gmail.com