IJPPP Copyright © 2009-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Int J Physiol Pathophysiol Pharmacol 2009;1(1):15-22.

Original Article
Influence of cimetidine and bromocriptine on prolactin levels in rat fertility

Qamar Hamid, Sadaf Hamid, Liaqat Ali Minhas, Anjuman Gul

Department of Anatomy, Dow university of health sciences, Karachi; Department of Anatomy, Ziauddin University, 4/B Shahra-e- Ghalib,
Clifton, Karachi-75600, Pakistan; Department of Anatomy, Army Medical College, Rawalpindi, Pakistan; Department of Biochemistry,
Ziauddin University, Clifton, Karachi-75600, Pakistan

Received  October 2, 2008, accepted and available online Nov 2008

Abstract: Objective: The present study was designed to see the effects of parenterally administered drugs cimetidine and
bromocriptine affecting serum prolactin upon the fertility of adult male albino rats. Ninety adult young male albino rats between the ages
of 60 to 120 days were selected. The animals were divided into three groups. Cimetidine was administered in a dose of 200 mg/kg
body weight to group B intramuscularly and in addition to cimetidine, bromocriptine in a dose of 2.5 mg/day intramuscularly was given
to group C. Normal saline was administered intramuscularly to control group A. Plasma prolactin was measured by Enzyme
Immunoassays. Spermatogonia, spermotocytes, and spermatids were studied under oil immersion. The final plasma prolactin level
instead of being elevated was found slightly depressed though insignificant in case of group B while remained slightly elevated instead
of being suppressed/depressed though insignificant in group C. In group B spermatogenesis was normal in almost all of the tubules
but a few of them were seen lined with only sertoli cells and all the other germ cells like spermatogonia, primary spermatocyes,
spermatids early and late, and spermatozoa were absent indicating total atrophy with both Sertoli cells and Leydig cells hyperplasia.
While in the moderately affected tubules different types of spermatogonia A/B or intermediate were seen near the basement
membrane. In group C both normal and abnormal germinal epithelium was seen in same/different tubules but a few of them were
seen lined with only sertoli cells and all the other germ cells like spermatogonia, primary spermatocytes, spermatids early and late,
and spermatozoa were absent. The process of spermatogenesis was variable and appeared to be normal in most but in some it was
found to be suppressed. This study revealed that the toxic effect of the drugs contributes to the infertility. It has not shown to be
mediated through hormones in present study for which further research work is needed using low dose and longer duration to see the
role of prolactin in causing infertility. (IJPPP810002).

Key words: Fertility, cimetidine, bromocriptine, prolactin

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Address all correspondence to:
Dr Sadaf Hamid,
Assistant professor,
Department of Anatomy,
Ziauddin University,
4/B Shahra-e- Ghalib,
Clifton, Karachi-75600, Pakistan.
Tel: 92-21-5862939
Fax: 9221-5862940
E-mail: sadafhamid2001@yahoo.co.in