Int J Physiol Pathophysiol Pharmacol 2009;1(1):64-75.
Original Article Versican isoforms modulate expression and function of nicotinic acetylcholine receptors
Yaojiong Wu, Wang Sheng, Haiheng Dong, David Lapierre, Yudi Wan, Wei-Yang Lu, Burton B. Yang
Sunnybrook Research Institute, Sunnybrook Health Sciences Centre; Department of Laboratory Medicine and Pathobiology, University of Toronto; Department of Anaesthesia, University of Toronto
Received February 8, 2009; accepted February 13, 2008; available online February 15, 2009
Abstract: Versican is a chondroitin sulfate proteoglycan whose isoforms are differentially expressed, but little is known of their functions in the neuronal system. Here we show that isoforms of versican play different roles in neuronal differentiation and neurite outgrowth. Expression of versican V1 isoform in PC12 cells induced complete neuronal differentiation and increased the expression of nicotinic acetylcholine receptor in NGF-independent manners. The V1-induced neuronal differentiation was different from the NGF-induced differentiation, showing a specific profile of nAChR subunit expression and distinct kinetics of receptor-gated channel activity. Our results have implications for understanding how versican regulates neuronal development, function and repair. (IJPPP902004).
Address all correspondence to: Wei-Yang Lu, MD, PhD Research Building, Sunnybrook Health Sciences Centre 2075 Bayview Avenue, Toronto M4N 3M5 Canada Tel: (416) 480-4823, E-mail: firstname.lastname@example.org
Burton B. Yang, MD, PhD, Research Building Sunnybrook Health Sciences Centre 2075 Bayview Avenue, Toronto M4N 3M5 Canada Tel: (416) 480-5874; E-mail: email@example.com